Real-time Polymerase Chain Reaction:Article Title: Transcriptional control of nucleus accumbens neuronal excitability by retinoid X receptor alpha tunes sensitivity to drug rewards.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies See Table S4 N/A N/A Anti-mouse peroxidase-conjugated secondary antibody Vector Laboratories CatPI-2000 Anti-rabbit peroxidase-conjugated secondary antibody Vector Laboratories Cat#PI-1000 Oligonucleotides See Table S5 for qPCR primers N/A N/A See method details for miRNA sequences N/A N/A FISH probe: Mm-Rxra ACD Bio Cat#63121 FISH probe: Mm-Drd1a-C2 ACD Bio Cat#406491-C2 FISH probe: Mm-Drd2-C3 ACD Bio Cat#406501-C3 Chemicals Cocaine HCl NIDA N/A Morphine SO4 NIDA N/A HX531 Tocris Cat#3912 PBS Thermo Fisher Scientific 14-190-144 DMSO Sigma D4540 CNO dihydrochloride Tocris Cat#6329 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Cat#000664 Mouse: Tg(Drd1-cre)FK150Gsat GENSAT MGI:3836633 Mouse: Tg(Drd2-cre)ER44Gsat GENSAT MGI:3836635 Mouse: Tg(Drd1-tdTomato)5Calak Shuen et al., 200869 MGI:4360387 Mouse: Rosa26fsTRAP The Jackson Laboratory IMSR_JAX:022367 Rat: SAS Sprague Dawley Charles River Cat#400 Viruses HSV-GFP Gene Delivery Core, MGH N/A HSV-RXRa-GFP Gene Delivery Core, MGH N/A AAV9-CAG-DIO-EGFP Viral Vector Core, Duke BK277 AAV9-CAG-DIO-EGFP-P2A-RXRa Viral Vector Core, Duke BK1432 AAV9-CMV-EYFP-miR-lacZ Viral Vector Core, Duke BK1202 AAV9-CMV-EYFP-miR-RXRa Viral Vector Core, Duke BK1149 AAV9-CMV-EGFP Wilson lab Addgene #105530 AAV9-CMV-EGFP-P2A-RXRa Viral Vector Core, Duke BK1418 AAV9-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al., 2011 Addgene #44361 AAV9-hSyn-DIO-mCherry Roth lab Addgene #50459 Software and algorithms FastQC v0.11.9 Babraham Institute RRID:SCR_014583 Trimmomatic v0.27 Usadel lab RRID:SCR_011848 hisat2 v2.1.0 Kim lab RRID:SCR_015530 Picard v2.18.10 Broad Institute RRID:SCR_006525 subread v2.0.0 N/A RRID:SCR_009803 DESeq2 v1.34.0 Love et al.70 RRID:SCR_015687 (Continued on next page) e1 Neuron 111, 1453–1467.e1–e7, May 3, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER RRHO2 Cahill et al., 201871 github.com/RRHO2/RRHO2 PANTHER Mi et al, 201972 pantherdb.org ComDet v0.5.4 N/A github.com/ekatrukha/ComDet Axon pCLAMP 11 Software Suite Molecular Devices RRID:SCR_011323 R v4.0.2 R foundation RRID:SCR_001905 Image J v1.53c NIH RRID:SCR_003070 Deposited data Raw snRNAseq data N/A GEO: GSE198527
Fluorescence In Situ Hybridization:Article Title: Transcriptional control of nucleus accumbens neuronal excitability by retinoid X receptor alpha tunes sensitivity to drug rewards.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies See Table S4 N/A N/A Anti-mouse peroxidase-conjugated secondary antibody Vector Laboratories CatPI-2000 Anti-rabbit peroxidase-conjugated secondary antibody Vector Laboratories Cat#PI-1000 Oligonucleotides See Table S5 for qPCR primers N/A N/A See method details for miRNA sequences N/A N/A FISH probe: Mm-Rxra ACD Bio Cat#63121 FISH probe: Mm-Drd1a-C2 ACD Bio Cat#406491-C2 FISH probe: Mm-Drd2-C3 ACD Bio Cat#406501-C3 Chemicals Cocaine HCl NIDA N/A Morphine SO4 NIDA N/A HX531 Tocris Cat#3912 PBS Thermo Fisher Scientific 14-190-144 DMSO Sigma D4540 CNO dihydrochloride Tocris Cat#6329 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Cat#000664 Mouse: Tg(Drd1-cre)FK150Gsat GENSAT MGI:3836633 Mouse: Tg(Drd2-cre)ER44Gsat GENSAT MGI:3836635 Mouse: Tg(Drd1-tdTomato)5Calak Shuen et al., 200869 MGI:4360387 Mouse: Rosa26fsTRAP The Jackson Laboratory IMSR_JAX:022367 Rat: SAS Sprague Dawley Charles River Cat#400 Viruses HSV-GFP Gene Delivery Core, MGH N/A HSV-RXRa-GFP Gene Delivery Core, MGH N/A AAV9-CAG-DIO-EGFP Viral Vector Core, Duke BK277 AAV9-CAG-DIO-EGFP-P2A-RXRa Viral Vector Core, Duke BK1432 AAV9-CMV-EYFP-miR-lacZ Viral Vector Core, Duke BK1202 AAV9-CMV-EYFP-miR-RXRa Viral Vector Core, Duke BK1149 AAV9-CMV-EGFP Wilson lab Addgene #105530 AAV9-CMV-EGFP-P2A-RXRa Viral Vector Core, Duke BK1418 AAV9-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al., 2011 Addgene #44361 AAV9-hSyn-DIO-mCherry Roth lab Addgene #50459 Software and algorithms FastQC v0.11.9 Babraham Institute RRID:SCR_014583 Trimmomatic v0.27 Usadel lab RRID:SCR_011848 hisat2 v2.1.0 Kim lab RRID:SCR_015530 Picard v2.18.10 Broad Institute RRID:SCR_006525 subread v2.0.0 N/A RRID:SCR_009803 DESeq2 v1.34.0 Love et al.70 RRID:SCR_015687 (Continued on next page) e1 Neuron 111, 1453–1467.e1–e7, May 3, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER RRHO2 Cahill et al., 201871 github.com/RRHO2/RRHO2 PANTHER Mi et al, 201972 pantherdb.org ComDet v0.5.4 N/A github.com/ekatrukha/ComDet Axon pCLAMP 11 Software Suite Molecular Devices RRID:SCR_011323 R v4.0.2 R foundation RRID:SCR_001905 Image J v1.53c NIH RRID:SCR_003070 Deposited data Raw snRNAseq data N/A GEO: GSE198527
Software:Article Title: Transcriptional control of nucleus accumbens neuronal excitability by retinoid X receptor alpha tunes sensitivity to drug rewards.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies See Table S4 N/A N/A Anti-mouse peroxidase-conjugated secondary antibody Vector Laboratories CatPI-2000 Anti-rabbit peroxidase-conjugated secondary antibody Vector Laboratories Cat#PI-1000 Oligonucleotides See Table S5 for qPCR primers N/A N/A See method details for miRNA sequences N/A N/A FISH probe: Mm-Rxra ACD Bio Cat#63121 FISH probe: Mm-Drd1a-C2 ACD Bio Cat#406491-C2 FISH probe: Mm-Drd2-C3 ACD Bio Cat#406501-C3 Chemicals Cocaine HCl NIDA N/A Morphine SO4 NIDA N/A HX531 Tocris Cat#3912 PBS Thermo Fisher Scientific 14-190-144 DMSO Sigma D4540 CNO dihydrochloride Tocris Cat#6329 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Cat#000664 Mouse: Tg(Drd1-cre)FK150Gsat GENSAT MGI:3836633 Mouse: Tg(Drd2-cre)ER44Gsat GENSAT MGI:3836635 Mouse: Tg(Drd1-tdTomato)5Calak Shuen et al., 200869 MGI:4360387 Mouse: Rosa26fsTRAP The Jackson Laboratory IMSR_JAX:022367 Rat: SAS Sprague Dawley Charles River Cat#400 Viruses HSV-GFP Gene Delivery Core, MGH N/A HSV-RXRa-GFP Gene Delivery Core, MGH N/A AAV9-CAG-DIO-EGFP Viral Vector Core, Duke BK277 AAV9-CAG-DIO-EGFP-P2A-RXRa Viral Vector Core, Duke BK1432 AAV9-CMV-EYFP-miR-lacZ Viral Vector Core, Duke BK1202 AAV9-CMV-EYFP-miR-RXRa Viral Vector Core, Duke BK1149 AAV9-CMV-EGFP Wilson lab Addgene #105530 AAV9-CMV-EGFP-P2A-RXRa Viral Vector Core, Duke BK1418 AAV9-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al., 2011 Addgene #44361 AAV9-hSyn-DIO-mCherry Roth lab Addgene #50459 Software and algorithms FastQC v0.11.9 Babraham Institute RRID:SCR_014583 Trimmomatic v0.27 Usadel lab RRID:SCR_011848 hisat2 v2.1.0 Kim lab RRID:SCR_015530 Picard v2.18.10 Broad Institute RRID:SCR_006525 subread v2.0.0 N/A RRID:SCR_009803 DESeq2 v1.34.0 Love et al.70 RRID:SCR_015687 (Continued on next page) e1 Neuron 111, 1453–1467.e1–e7, May 3, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER RRHO2 Cahill et al., 201871 github.com/RRHO2/RRHO2 PANTHER Mi et al, 201972 pantherdb.org ComDet v0.5.4 N/A github.com/ekatrukha/ComDet Axon pCLAMP 11 Software Suite Molecular Devices RRID:SCR_011323 R v4.0.2 R foundation RRID:SCR_001905 Image J v1.53c NIH RRID:SCR_003070 Deposited data Raw snRNAseq data N/A GEO: GSE198527
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